Review



ib4  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    Vector Laboratories ib4
    Tmem45b is primarily expressed in itch-sensing neurons. (A) Immunostaining results show the expression of Tmem45b (green), CGRP (red), and NF200 (red). (B) Immunostaining results show the co-expression of Tmem45b (green) and <t>IB4</t> (red). (C) Statistical analysis shows the proportion of Tmem45b + IB4 + . (D) scRNA-seq (10x Genomics) analysis identified 16 subtypes of DRG neurons. Gene annotations for these 16 neuron types are shown in the right panel. Tmem45b is predominantly enriched in neurons positive for Mrgprd, Mrgpra3, Nppb , and Th . (E) RNAscope in situ hybridization shows co-localization among Tmem45b (green), Mrgprd (red) , Mrgpra3 (red), and Nppb (red). Immunostaining results show that Tmem45b (green) is co-expressed with Th (red). Arrows indicate co-expressing cells. Scale bar, 20 μm. (F) Proportions of Mrgprd + , Mrgpra3 + , Nppb + , and Th + neurons among Tmem45b + DRG neurons. DRG was obtained from at least 3 mice.
    Ib4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ib4/product/Vector Laboratories
    Average 95 stars, based on 323 article reviews
    ib4 - by Bioz Stars, 2026-03
    95/100 stars

    Images

    1) Product Images from "Tmem45b modulates itch via endoplasmic reticulum calcium regulation"

    Article Title: Tmem45b modulates itch via endoplasmic reticulum calcium regulation

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2025.1708686

    Tmem45b is primarily expressed in itch-sensing neurons. (A) Immunostaining results show the expression of Tmem45b (green), CGRP (red), and NF200 (red). (B) Immunostaining results show the co-expression of Tmem45b (green) and IB4 (red). (C) Statistical analysis shows the proportion of Tmem45b + IB4 + . (D) scRNA-seq (10x Genomics) analysis identified 16 subtypes of DRG neurons. Gene annotations for these 16 neuron types are shown in the right panel. Tmem45b is predominantly enriched in neurons positive for Mrgprd, Mrgpra3, Nppb , and Th . (E) RNAscope in situ hybridization shows co-localization among Tmem45b (green), Mrgprd (red) , Mrgpra3 (red), and Nppb (red). Immunostaining results show that Tmem45b (green) is co-expressed with Th (red). Arrows indicate co-expressing cells. Scale bar, 20 μm. (F) Proportions of Mrgprd + , Mrgpra3 + , Nppb + , and Th + neurons among Tmem45b + DRG neurons. DRG was obtained from at least 3 mice.
    Figure Legend Snippet: Tmem45b is primarily expressed in itch-sensing neurons. (A) Immunostaining results show the expression of Tmem45b (green), CGRP (red), and NF200 (red). (B) Immunostaining results show the co-expression of Tmem45b (green) and IB4 (red). (C) Statistical analysis shows the proportion of Tmem45b + IB4 + . (D) scRNA-seq (10x Genomics) analysis identified 16 subtypes of DRG neurons. Gene annotations for these 16 neuron types are shown in the right panel. Tmem45b is predominantly enriched in neurons positive for Mrgprd, Mrgpra3, Nppb , and Th . (E) RNAscope in situ hybridization shows co-localization among Tmem45b (green), Mrgprd (red) , Mrgpra3 (red), and Nppb (red). Immunostaining results show that Tmem45b (green) is co-expressed with Th (red). Arrows indicate co-expressing cells. Scale bar, 20 μm. (F) Proportions of Mrgprd + , Mrgpra3 + , Nppb + , and Th + neurons among Tmem45b + DRG neurons. DRG was obtained from at least 3 mice.

    Techniques Used: Immunostaining, Expressing, RNAscope, In Situ Hybridization



    Similar Products

    ib4  (Vector Laboratories)
    95
    Vector Laboratories ib4
    Tmem45b is primarily expressed in itch-sensing neurons. (A) Immunostaining results show the expression of Tmem45b (green), CGRP (red), and NF200 (red). (B) Immunostaining results show the co-expression of Tmem45b (green) and <t>IB4</t> (red). (C) Statistical analysis shows the proportion of Tmem45b + IB4 + . (D) scRNA-seq (10x Genomics) analysis identified 16 subtypes of DRG neurons. Gene annotations for these 16 neuron types are shown in the right panel. Tmem45b is predominantly enriched in neurons positive for Mrgprd, Mrgpra3, Nppb , and Th . (E) RNAscope in situ hybridization shows co-localization among Tmem45b (green), Mrgprd (red) , Mrgpra3 (red), and Nppb (red). Immunostaining results show that Tmem45b (green) is co-expressed with Th (red). Arrows indicate co-expressing cells. Scale bar, 20 μm. (F) Proportions of Mrgprd + , Mrgpra3 + , Nppb + , and Th + neurons among Tmem45b + DRG neurons. DRG was obtained from at least 3 mice.
    Ib4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ib4/product/Vector Laboratories
    Average 95 stars, based on 1 article reviews
    ib4 - by Bioz Stars, 2026-03
    95/100 stars
    		  Buy from Supplier
    biotin conjugated ib4  (Thermo Fisher)
    99
    Thermo Fisher biotin conjugated ib4
    Tmem45b is primarily expressed in itch-sensing neurons. (A) Immunostaining results show the expression of Tmem45b (green), CGRP (red), and NF200 (red). (B) Immunostaining results show the co-expression of Tmem45b (green) and <t>IB4</t> (red). (C) Statistical analysis shows the proportion of Tmem45b + IB4 + . (D) scRNA-seq (10x Genomics) analysis identified 16 subtypes of DRG neurons. Gene annotations for these 16 neuron types are shown in the right panel. Tmem45b is predominantly enriched in neurons positive for Mrgprd, Mrgpra3, Nppb , and Th . (E) RNAscope in situ hybridization shows co-localization among Tmem45b (green), Mrgprd (red) , Mrgpra3 (red), and Nppb (red). Immunostaining results show that Tmem45b (green) is co-expressed with Th (red). Arrows indicate co-expressing cells. Scale bar, 20 μm. (F) Proportions of Mrgprd + , Mrgpra3 + , Nppb + , and Th + neurons among Tmem45b + DRG neurons. DRG was obtained from at least 3 mice.
    Biotin Conjugated Ib4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin conjugated ib4/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    biotin conjugated ib4 - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    anti ib4 fitc  (Vector Laboratories)
    94
    Vector Laboratories anti ib4 fitc
    Tmem45b is primarily expressed in itch-sensing neurons. (A) Immunostaining results show the expression of Tmem45b (green), CGRP (red), and NF200 (red). (B) Immunostaining results show the co-expression of Tmem45b (green) and <t>IB4</t> (red). (C) Statistical analysis shows the proportion of Tmem45b + IB4 + . (D) scRNA-seq (10x Genomics) analysis identified 16 subtypes of DRG neurons. Gene annotations for these 16 neuron types are shown in the right panel. Tmem45b is predominantly enriched in neurons positive for Mrgprd, Mrgpra3, Nppb , and Th . (E) RNAscope in situ hybridization shows co-localization among Tmem45b (green), Mrgprd (red) , Mrgpra3 (red), and Nppb (red). Immunostaining results show that Tmem45b (green) is co-expressed with Th (red). Arrows indicate co-expressing cells. Scale bar, 20 μm. (F) Proportions of Mrgprd + , Mrgpra3 + , Nppb + , and Th + neurons among Tmem45b + DRG neurons. DRG was obtained from at least 3 mice.
    Anti Ib4 Fitc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ib4 fitc/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    anti ib4 fitc - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    biotinylated anti ib4  (Vector Laboratories)
    95
    Vector Laboratories biotinylated anti ib4
    Tmem45b is primarily expressed in itch-sensing neurons. (A) Immunostaining results show the expression of Tmem45b (green), CGRP (red), and NF200 (red). (B) Immunostaining results show the co-expression of Tmem45b (green) and <t>IB4</t> (red). (C) Statistical analysis shows the proportion of Tmem45b + IB4 + . (D) scRNA-seq (10x Genomics) analysis identified 16 subtypes of DRG neurons. Gene annotations for these 16 neuron types are shown in the right panel. Tmem45b is predominantly enriched in neurons positive for Mrgprd, Mrgpra3, Nppb , and Th . (E) RNAscope in situ hybridization shows co-localization among Tmem45b (green), Mrgprd (red) , Mrgpra3 (red), and Nppb (red). Immunostaining results show that Tmem45b (green) is co-expressed with Th (red). Arrows indicate co-expressing cells. Scale bar, 20 μm. (F) Proportions of Mrgprd + , Mrgpra3 + , Nppb + , and Th + neurons among Tmem45b + DRG neurons. DRG was obtained from at least 3 mice.
    Biotinylated Anti Ib4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti ib4/product/Vector Laboratories
    Average 95 stars, based on 1 article reviews
    biotinylated anti ib4 - by Bioz Stars, 2026-03
    95/100 stars
    		  Buy from Supplier
    anti ib4  (Vector Laboratories)
    95
    Vector Laboratories anti ib4
    Tmem45b is primarily expressed in itch-sensing neurons. (A) Immunostaining results show the expression of Tmem45b (green), CGRP (red), and NF200 (red). (B) Immunostaining results show the co-expression of Tmem45b (green) and <t>IB4</t> (red). (C) Statistical analysis shows the proportion of Tmem45b + IB4 + . (D) scRNA-seq (10x Genomics) analysis identified 16 subtypes of DRG neurons. Gene annotations for these 16 neuron types are shown in the right panel. Tmem45b is predominantly enriched in neurons positive for Mrgprd, Mrgpra3, Nppb , and Th . (E) RNAscope in situ hybridization shows co-localization among Tmem45b (green), Mrgprd (red) , Mrgpra3 (red), and Nppb (red). Immunostaining results show that Tmem45b (green) is co-expressed with Th (red). Arrows indicate co-expressing cells. Scale bar, 20 μm. (F) Proportions of Mrgprd + , Mrgpra3 + , Nppb + , and Th + neurons among Tmem45b + DRG neurons. DRG was obtained from at least 3 mice.
    Anti Ib4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ib4/product/Vector Laboratories
    Average 95 stars, based on 1 article reviews
    anti ib4 - by Bioz Stars, 2026-03
    95/100 stars
    		  Buy from Supplier
    dylight 594 griffonia simplicifolia lectin isolectin b4 ib4  (Vector Laboratories)
    94
    Vector Laboratories dylight 594 griffonia simplicifolia lectin isolectin b4 ib4
    Tmem45b is primarily expressed in itch-sensing neurons. (A) Immunostaining results show the expression of Tmem45b (green), CGRP (red), and NF200 (red). (B) Immunostaining results show the co-expression of Tmem45b (green) and <t>IB4</t> (red). (C) Statistical analysis shows the proportion of Tmem45b + IB4 + . (D) scRNA-seq (10x Genomics) analysis identified 16 subtypes of DRG neurons. Gene annotations for these 16 neuron types are shown in the right panel. Tmem45b is predominantly enriched in neurons positive for Mrgprd, Mrgpra3, Nppb , and Th . (E) RNAscope in situ hybridization shows co-localization among Tmem45b (green), Mrgprd (red) , Mrgpra3 (red), and Nppb (red). Immunostaining results show that Tmem45b (green) is co-expressed with Th (red). Arrows indicate co-expressing cells. Scale bar, 20 μm. (F) Proportions of Mrgprd + , Mrgpra3 + , Nppb + , and Th + neurons among Tmem45b + DRG neurons. DRG was obtained from at least 3 mice.
    Dylight 594 Griffonia Simplicifolia Lectin Isolectin B4 Ib4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dylight 594 griffonia simplicifolia lectin isolectin b4 ib4/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    dylight 594 griffonia simplicifolia lectin isolectin b4 ib4 - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    ib4 lectin  (Vector Laboratories)
    95
    Vector Laboratories ib4 lectin
    Selection of homozygously edited cells based on highest surrogate reporter expression. ( A ) Schematic of selection strategy using version 2.1- eGFP (V2.1G), a nonintegrated surrogate reporter system. PFFs were cotransfected with V2.1G, Cas9, and sgRNA plasmid, whereas the control group was only transfected with the V2.1G plasmid. FACS analysis revealed varying intensities of FITC expression among the cells. (H) The highest FITC intensity population, (M) the middle FITC intensity population, (L) the lower FITC intensity population, and (N) the negative FITC intensity population. ( B ) Correlation between genome editing efficiency and FITC intensity of endogenous targeting in PFFs. ( C ) Representative Sanger sequences of CMAH target site in wild-type cells, homozygous cell clones, and cell pools selected by V2.1G. ( D ) FACS analysis of GGTA1 targeting in PFF pools detection by <t>IB4</t> <t>lectin</t> staining. ( E ) Western blot analysis of USE1-targeted Vero E6 cell pools with middle and high FITC intensity, respectively.
    Ib4 Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ib4 lectin/product/Vector Laboratories
    Average 95 stars, based on 1 article reviews
    ib4 lectin - by Bioz Stars, 2026-03
    95/100 stars
    		  Buy from Supplier
    anti ib4 alexa fluor 488 conjugate  (Thermo Fisher)
    90
    Thermo Fisher anti ib4 alexa fluor 488 conjugate
    Selection of homozygously edited cells based on highest surrogate reporter expression. ( A ) Schematic of selection strategy using version 2.1- eGFP (V2.1G), a nonintegrated surrogate reporter system. PFFs were cotransfected with V2.1G, Cas9, and sgRNA plasmid, whereas the control group was only transfected with the V2.1G plasmid. FACS analysis revealed varying intensities of FITC expression among the cells. (H) The highest FITC intensity population, (M) the middle FITC intensity population, (L) the lower FITC intensity population, and (N) the negative FITC intensity population. ( B ) Correlation between genome editing efficiency and FITC intensity of endogenous targeting in PFFs. ( C ) Representative Sanger sequences of CMAH target site in wild-type cells, homozygous cell clones, and cell pools selected by V2.1G. ( D ) FACS analysis of GGTA1 targeting in PFF pools detection by <t>IB4</t> <t>lectin</t> staining. ( E ) Western blot analysis of USE1-targeted Vero E6 cell pools with middle and high FITC intensity, respectively.
    Anti Ib4 Alexa Fluor 488 Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ib4 alexa fluor 488 conjugate/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    anti ib4 alexa fluor 488 conjugate - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    ib4 lectin 509  (Vector Laboratories)
    95
    Vector Laboratories ib4 lectin 509
    Selection of homozygously edited cells based on highest surrogate reporter expression. ( A ) Schematic of selection strategy using version 2.1- eGFP (V2.1G), a nonintegrated surrogate reporter system. PFFs were cotransfected with V2.1G, Cas9, and sgRNA plasmid, whereas the control group was only transfected with the V2.1G plasmid. FACS analysis revealed varying intensities of FITC expression among the cells. (H) The highest FITC intensity population, (M) the middle FITC intensity population, (L) the lower FITC intensity population, and (N) the negative FITC intensity population. ( B ) Correlation between genome editing efficiency and FITC intensity of endogenous targeting in PFFs. ( C ) Representative Sanger sequences of CMAH target site in wild-type cells, homozygous cell clones, and cell pools selected by V2.1G. ( D ) FACS analysis of GGTA1 targeting in PFF pools detection by <t>IB4</t> <t>lectin</t> staining. ( E ) Western blot analysis of USE1-targeted Vero E6 cell pools with middle and high FITC intensity, respectively.
    Ib4 Lectin 509, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ib4 lectin 509/product/Vector Laboratories
    Average 95 stars, based on 1 article reviews
    ib4 lectin 509 - by Bioz Stars, 2026-03
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Tmem45b is primarily expressed in itch-sensing neurons. (A) Immunostaining results show the expression of Tmem45b (green), CGRP (red), and NF200 (red). (B) Immunostaining results show the co-expression of Tmem45b (green) and IB4 (red). (C) Statistical analysis shows the proportion of Tmem45b + IB4 + . (D) scRNA-seq (10x Genomics) analysis identified 16 subtypes of DRG neurons. Gene annotations for these 16 neuron types are shown in the right panel. Tmem45b is predominantly enriched in neurons positive for Mrgprd, Mrgpra3, Nppb , and Th . (E) RNAscope in situ hybridization shows co-localization among Tmem45b (green), Mrgprd (red) , Mrgpra3 (red), and Nppb (red). Immunostaining results show that Tmem45b (green) is co-expressed with Th (red). Arrows indicate co-expressing cells. Scale bar, 20 μm. (F) Proportions of Mrgprd + , Mrgpra3 + , Nppb + , and Th + neurons among Tmem45b + DRG neurons. DRG was obtained from at least 3 mice.

    Journal: Frontiers in Physiology

    Article Title: Tmem45b modulates itch via endoplasmic reticulum calcium regulation

    doi: 10.3389/fphys.2025.1708686

    Figure Lengend Snippet: Tmem45b is primarily expressed in itch-sensing neurons. (A) Immunostaining results show the expression of Tmem45b (green), CGRP (red), and NF200 (red). (B) Immunostaining results show the co-expression of Tmem45b (green) and IB4 (red). (C) Statistical analysis shows the proportion of Tmem45b + IB4 + . (D) scRNA-seq (10x Genomics) analysis identified 16 subtypes of DRG neurons. Gene annotations for these 16 neuron types are shown in the right panel. Tmem45b is predominantly enriched in neurons positive for Mrgprd, Mrgpra3, Nppb , and Th . (E) RNAscope in situ hybridization shows co-localization among Tmem45b (green), Mrgprd (red) , Mrgpra3 (red), and Nppb (red). Immunostaining results show that Tmem45b (green) is co-expressed with Th (red). Arrows indicate co-expressing cells. Scale bar, 20 μm. (F) Proportions of Mrgprd + , Mrgpra3 + , Nppb + , and Th + neurons among Tmem45b + DRG neurons. DRG was obtained from at least 3 mice.

    Article Snippet: Antibodies used in this study: GM130 (BD, 610822), Th (Millipore, AB1542), IB4 (Vector, FL-1201-.5), Tuj1 (Starter, SDT-251-28), CGRP (Dia Sorin, 24112), NF200 (CST, 2836S), PDI (Santa Cruz, SC-20132), Calnexin (Abcam, ab112995), Serca1 (Proteintech, 22361-1-AP), mitochondria-tracer (Beyotime, C1048), TGN38 (Bio-Rad, AHP499G), GFAP (Millipore, MAB3402), IBA1 (Abcam, ab5076).

    Techniques: Immunostaining, Expressing, RNAscope, In Situ Hybridization

    Selection of homozygously edited cells based on highest surrogate reporter expression. ( A ) Schematic of selection strategy using version 2.1- eGFP (V2.1G), a nonintegrated surrogate reporter system. PFFs were cotransfected with V2.1G, Cas9, and sgRNA plasmid, whereas the control group was only transfected with the V2.1G plasmid. FACS analysis revealed varying intensities of FITC expression among the cells. (H) The highest FITC intensity population, (M) the middle FITC intensity population, (L) the lower FITC intensity population, and (N) the negative FITC intensity population. ( B ) Correlation between genome editing efficiency and FITC intensity of endogenous targeting in PFFs. ( C ) Representative Sanger sequences of CMAH target site in wild-type cells, homozygous cell clones, and cell pools selected by V2.1G. ( D ) FACS analysis of GGTA1 targeting in PFF pools detection by IB4 lectin staining. ( E ) Western blot analysis of USE1-targeted Vero E6 cell pools with middle and high FITC intensity, respectively.

    Journal: Genome Research

    Article Title: Homozygous editing of multiple genes for accelerated generation of xenotransplantation pigs

    doi: 10.1101/gr.279709.124

    Figure Lengend Snippet: Selection of homozygously edited cells based on highest surrogate reporter expression. ( A ) Schematic of selection strategy using version 2.1- eGFP (V2.1G), a nonintegrated surrogate reporter system. PFFs were cotransfected with V2.1G, Cas9, and sgRNA plasmid, whereas the control group was only transfected with the V2.1G plasmid. FACS analysis revealed varying intensities of FITC expression among the cells. (H) The highest FITC intensity population, (M) the middle FITC intensity population, (L) the lower FITC intensity population, and (N) the negative FITC intensity population. ( B ) Correlation between genome editing efficiency and FITC intensity of endogenous targeting in PFFs. ( C ) Representative Sanger sequences of CMAH target site in wild-type cells, homozygous cell clones, and cell pools selected by V2.1G. ( D ) FACS analysis of GGTA1 targeting in PFF pools detection by IB4 lectin staining. ( E ) Western blot analysis of USE1-targeted Vero E6 cell pools with middle and high FITC intensity, respectively.

    Article Snippet: The following lectins and antibodies were used for staining: IB4 lectin (Vector FL-1201, 1:50 dilution), DBA lectin (Vector FL-1031, 1:50 dilution), AntiNeuGC (BioLegend 146901, 1:50 dilution), and thrombomodulin antibody (Abmart T55279, 1:100 dilution).

    Techniques: Selection, Expressing, Plasmid Preparation, Control, Transfection, Clone Assay, Staining, Western Blot